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KMID : 0613820120220121688
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Journal of Life Science
2012 Volume.22 No. 12 p.1688 ~ p.1696
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Inhibition of Adipocyte Differentiation by Methanol Extracts of Oenanthe javanica Seed in 3T3-L1 Preadipocytes
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Ji Hyang-Hwa
Jeong Hyun-Young
Jin Soo-Jung
Kwon Hyun-Ju
Kim Byung-Woo
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Abstract
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Oenanthe javanica has been used as a food source and also in traditional folk medicine for its detoxifying properties and anti-microbial effects since ancient times. In this study, we evaluated the effect and mechanism of O. javanica seed methanol extract (OJSE) on adipocyte differentiation by 3T3-L1 preadipocytes. Under non-toxic conditions, OJSE treatment resulted in a dose-dependent inhibition of lipid droplet generation and triglyceride accumulation by suppressing adipocyte differentiation, which are associated with the decreased expression of key proadipogenic transcription factors including CCAAR/enhancer binding protein α, β (C/EBPα, C/EBPβ) and peroxisome proliferator-activated receptor r (PPARr). OJSE also significantly inhibited proliferation and differentiation of 3T3-L1 preadipocytes through G1-phase arrest, indicating that OJSE blocked mitotic clonal expansion during adipocyte differentiation. Investigation of the alteration of G1 phase arrest-related proteins indicated a dose-dependent increase in the expression of p21 and reduction in expression of cyclin E, Cdk2, E2F-1 and phospho-Rb by OSJE. Taken together, these results suggest that OJSE inhibits adipocyte differentiation by blocking the mitotic clonal expansion, which is accompanied by preadipocyte cell cycle arrest.
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KEYWORD
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Oenanthe javanica seed, adipocyte differentiation, CCAAT/enhancer binding proteins, peroxisome proliferator-activated receptor-r, mitotic clonal expansion
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